sequence results for cbx3 cloned into pJET and smc straight from plasmid pool 8-28-13
2014-08-29++ sequence results for cbx3 cloned into p JET and smc straight from plasmid pool 8-28-13
sequence results found here
"F:\kurt\storage\CIM Research Folder\DR\2013\8-28-13\dna sequence 8-28-13"
None of the smc sequences came back good.
Corresponding gel for cbx3 pcrs on 8-20-13.
Sequencing of smc sequences were straight from the plasmid pool, and this did not come back good.
What if I use Primer-BLAST with the FCX3N and RCX3N primers with the Hnrnpul1 transcript that came up so much?
Primer-Blast gave the following message:
Specified left primer cannot be found in template...make sure this primer is on the plus strand of your template.
I tried the Primer-BLAST with the Cbx3 transcript, and there were many side products. However, non of them were Hnrnpul1.