search 4-26-12
2014-08-29azim58 - search 4-26-12
tumor antigens nitrocellulose array
SEROLOGIC ANALYSIS OF OVARIAN TUMOR ANTIGENS REVEALS A BIAS TOWARD
ANTIGENS ENCODED ON 17q
by Institute for Systems Biology in Seattle with other Seattle groups
(2003)
they used sera from 25 ovarian cancer patients to screen 3 independent
cDNA libraries. They found 9 antigens. 4 of these antigens were in the
17q chromosome region.
Their cDNA library was in the form of phage
sera was precleared of E. coli-specific antibodies using an E. coli
affinity resin 5Prime-3Prime
E-mail conversation about this 5-4-12
plaques were originally screened in a low throughput way. They did use an
array-based secondary screening method though. However, this array was a
6X8 array. They made it so they could reproducibly keep screening a lot
of different sera.
Antibody detection using tumor-associated antigen mini-array in
immunodiagnosing human hepatocellular carcinoma
by Scripps Research Institute and many other universities in 2006
they just screen sera against a panel of 8 antigens. They just performed
ELISA, and used it mostly for diagnoses.
Serological identification of embryonic neural proteins as highly
immunogenic tumor antigens in small cell lung cancer
Ludwig Institute for Cancer Research in New York
SEREX against small cell lung cancer cell lines. They found embryonic
genes in the tumors
their tumor cDNA was expressed on phage
Serological and proteomic evaluation of antibody responses in the
identification of tumor antigens in renal cell carcinoma
by St. James's University Hospital in UK in 2003
they use a SPEAR approach which combines 2D polyacrylamide gel
electrophoresis of tumor samples with immunoblotting and protein
identification by mass spectrometry
original SEREX paper in 1995
Human neoplasms elicit multiple specific immune responses in the
autologous host
by Pfreundschuh in University of Searland Medical School in Homburg
Germany
they expressed tumor cDNA libraries in phage that infected E. coli and
screened with autologous patient serum. They discovered at least 4
antigens this way
there was much more antibody reactivity than they expected
they screened their library in a low-throughput nitrocellulose membrane
manner
Cellular and humoral immune responses to a human pancreatic cancer
antigen, coactosin-like protein, originally defined by the SEREX method
by Kumamoto University in Japan
quote: Cellular and humoral immune responses to a human pancreatic cancer
antigen, coactosin-like protein, originally defined by the SEREX method
they looked for pancreatic cancer antigens
they focus on coactosin-like protein CLP
they did their SEREX using the standard plate method
Identification of tumor-associated antigens in chronic lymphocytic
leukemia by SEREX
by Dana Farber Cancer Institute at Harvard and Benjamin Franklin
Universiyt in Berlin Germany
they cloned the tumor cDNA library into phage
they use nitrocellulose membranes
new search: high throughput serex
Highthroughput proteomic strategies for identifying tumour-associated
antigens
mentiones SERPA (2D electropheresis followed by mass spec)
Identification of prostate cancer antigens by automated high-throughput
filter immunoscreening
their method has 18,000 clones
they use phage for tumor cDNA library