scFv

2014-08-29

azim58 - scFv

scFv stands for single chain fragment variable. This name is derived from
the fact that an scFv is produced by combining the light and heavy chain
variable regions of an antibody into a single chain. The heavy and light
variable regions are combined by a linker.

Questions about scFv:

How long is a typical scFv?

o The VH,VL and ScFv DNAs were about 340 bp,. 320 bp and 750 bp
respectively.
- www.wjgnet.com/downpdf.asp?url=/1007-9327/8/258

What is the linker used in my scFv as of 1-5-12?

o scFv Phage Linker 1-5-12

===========================================================================
support that scFv can bind to the same epitopes as the original
antibodies can be found in this presentation:
"L:\storage\CIM Research Folder\kwhittem\Presentations\2010\10-20-10
Cancer Presentation\Cancer Meeting Presentation 102010.ppt"