questions about ordering random nucleotide oligos
2014-08-29azim58 - questions about ordering random nucleotide oligos
2-13-13
contacted SIGMA about ordering random oligos
my message
Is it more expensive to order oligos with random nucleotides as compared
with oligos with non-random nucleotides? How large would the price
difference be? Also, someone told me that in the past it was not possible
to order oligos with a random nucleotide at the 3' end of the oligo? Is
this still the case or can I order an oligo with a random nucleotide at
any position? I am asking these questions because I think I will soon
order random pentadecamers (15 bp oligos) to help me construct a cDNA
library. Thanks for any help and information you have to offer me!
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message back from SIGMA
Good Afternoon Kurt,
You can order a degenerative base at any position in you sequence. There
is no premium in pricing for the degenerative bases unless you would like
a unique mixture. You should be able to place the order online with no
issues. The price for a desalted oligo should cost about $4.80. If you
have any questions or concerns please let me know.
Regards,
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see message here
https://mail.google.com/mail/u/0/?ui=2&shva=1#label/Career/13cd4d76e7c7
9666