progress towards sequencing tmem fragment 6-25-13

2014-08-29

azim58 - progress towards sequencing tmem fragment 6-25-13


Before I perform 96 minipreps on one of the thap2+tmem19 (TR_E10339)
positive pools in the 1-41 pool set, I need to see if the sequence I have
would produce the same peptide that Shen has sera against.

According to the table found here

"F:\kurt\storage\CIM Research Folder\DR\2013\6-19-13\chim transc\table of
results 6-19-13.xlsx"
10339 was positive in pools 8, 19, and 20

I performed a PCR with dynazyme with 10339 primers with 8, 19, and 20
pools