poly dT primer portal 2-1-13
2014-08-29azim58 - poly dT primer portal 2-1-13
The purpose of a poly dT primer would be to amplify poly A mRNA. One
might want to make 1st strand cDNA from RNA, and then 2nd strand cDNA
from 1st strand cDNA.
Here are some primers I will order.
dT1
poly T primer for in-fusion with vector in directional SMARTer In-Fusion
kit
5’–CGGGGTACGATGAGACACCATTTTTTTTTTTTTTTTTTTTVN–3’
dT2
2nd strand synthesis primer for in-fusion with vector in directional
SMARTer In-Fusion kit
5’–CGGGGTACGATGAGACACCATTTTTTTTTTTTTTTTTTTT–3’
dT3
poly T primer (this could be used for 1st and 2nd strand synthesis
5'-TTTTTTTTTTTTTTTTTTTTVN–3’
"C:\kurt\storage\CIM Research Folder\DR\2013\2-1-13\poly dT primer\Primer
order form Kurt 2-1-13.xls"