pIII protein of M13 phage

2014-08-29

azim58 - pIII protein of M13 phage

There are typically 3-5 copies of pIII at one end of the phage

whereas there are many pVIII proteins all around the virus

The pIII protein appears to bind directly to phage. Note that I am

not completely sure if phi 29 phage works the same as m13 phage in this
regard.
o http://www.pnas.org/content/77/11/6425.full.pdf
- In this paper, we show that protein p3 is linked to the 5'
termini of 029 DNA through a phosphoester bond between serine and
5'-dAMP. A
preliminary account of some of these findings has been pre-sented

o 1. Protein p3 is linked to the DNA of phage 029 through a
phosphoester bond between serine and 5'-dAMP (initiation of
replication/Bacillus subtilis/nucleotidyl-peptide/acid and alkali
hydrolysis). JosE M. HERMOSO AND MARGARITA SALAS Centro de Biologla
Molecular (CSIGUAM), Universidad Autonoma, Canto Blanco, Madrid-34,
Spain
o
2. Gene 1:25-36 Sambrook, J. & Russell, D. (2001) Molecular Cloning:
A Laboratory Manual, Third Edition, (Cold Spring Harbor Laboratory
Press, Cold Spring Harbor, NY).