is it possible to nanodrop 1st strand cDNA_

2013-12-01

azim58 - is it possible to nanodrop 1st strand cDNA?


It looks like it is not a good idea to nanodrop 1st strand cDNA since
there will be leftover dNTPs from the reaction. Instead, one can use the
amount of RNA used to estimate the amount of 1st strand cDNA present.

see forum here:
http://www.researchgate.net/post/Quantification_of_cDNA