insert to vector ratios with ligation
2013-12-01azim58 - insert to vector ratios with ligation
Ligation vector and insert ratios
lab manuals usually say "molar ratio 1:3 vector: insert"
http://www.methods.info/Methods/RNA_DNA/ligation_simple.html
In most cases either a 1:1 or a 1:3 molar ratio of vector:insert works
well.
http://www.mcdb.ucla.edu/Research/Arispe/Protocols/Vector_Insert_Ratio.pdf
Here's a calculator for the insert to vector ratio problem
http://www.promega.com/techserv/tools/biomath/calc06.htm
Reasoning for vector to insert ratios
While if you increase the amount of insert, there is higher chance it
gots incorporated.
http://www.biology-online.org/biology-forum/about19798.html