forum response 3-22-12.1640
2013-12-01azim58 - forum response 3-22-12.1640
Hello,
We have just started doing ELISPOTS with protein instead of peptides.
Should we be allowing more time for the cells to respond to allow for
antigen processing and presentation? If so how long should we incubate
the cells for?
Thanks for your help.
reply
Subject protein vs.peptide
Date Wed Dec 19 2007 18:16
Author Peter Mellings ([email protected])
Yes. Insted of 20 hours at least 40 hours. There is a paper out by Carmen
Scheibenbogen's group in JIM: Schmittel A, Keilholz U, Scheibenbogen C.
Evaluation of the interferon-gamma ELISPOT-assay for quantification of
peptide specific T lymphocytes from peripheral blood.
J Immunol Methods. 1997 Dec 29;210(2):167-74.