determining concentration of dna
2013-12-01azim58 - determining concentration of dna
Nanodrop
Does glycogen absorb in the DNA range?
Not really very much. I did a test measurement of a 10 uL sample
containing 2 uL of 20 ug/uL glycogen and the result was 12.3 ng/uL with a
260/280 of 2.14. The curve also did not have a very high well shaped peak.
Also, the SMARTer In-Fusion directional library kit instructs the user to
nanodrop a sample after an ethanol precipitation with glycogen has been
performed