What transcripts are found in a human cell_ - Genome Biology

2015-01-13

azim58 - What transcripts are found in a human cell? - Genome Biology


What transcripts are found in a human cell? - Genome Biology

Genome Biology 2000, 1:reports031

doi:10.1186/gb-2000-1-1-reports031

http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&a
mp;cd=8&cad=rja&ved=0CHoQFjAH&url=http%3A%2F%2Fgenomebiology.co
m%2F2000%2F1%2F1%2Freports%2F031&ei=dHEuUf6OOeKCjALb4YD4BQ&usg=AFQj
CNFO6J8vQkRE_GScYRikJRk7miIFyg&sig2=-GTaPKmyoOwIpsiAvY0fUQ

q
An alternative to the much-hyped cDNA microarray technology for this
purpose is ... The average number of different but related transcripts
corresponding to each ... More than 43,000 transcripts were expressed in
a single cell type ... 500 copies per cell made up 18% of the cellular
mRNA mass (Figure 1a)


This short document mostly compares the EST technique to the SAGE
technique. Some of the information may be outdated since they estimated
the number of human genes in the genome to be about 84,000. The paper
looks like it has some very useful information about RNA though.

large number of transcripts that are identified as being present at fewer
than five copies per cell. These rare transcripts comprise 25% of
cellular mRNA by mass but 94% of unique transcripts

this paper has some good diagrams showing how most of the unique
transcripts occur at lower than 5 copies per cell


More than 43,000 transcripts were expressed in a single cell type <- I
don't know if this is the number of unique transcripts or total
transcripts

with expression levels ranging from 0.3 to 9,417 copies of the transcript
per cell.