Verify ligation of rcomptt frag 10-26-12
2015-01-13azim58 - Verify ligation of rcomptt frag 10-26-12
Verify ligation of rcomptt frag 10-26-12
Chose primers
"C:\kurt\storage\CIM Research Folder\DR\2012\10-26-12\rcompTT ver\pcomb
primer pick 10-26-12.SPF"
forward primer
SAPTFC
AGCGCCCAATACGCAAACCG
reverse primer
SRNotI
CGATTTCGGCCTATTGGTTA
see Numbers associated with pcomb rcomp ligation 10-26-1210
If the rcomp fragment is not present then the pcr product should be
284+1677+732 = 2693
If the rcomp fragment is present then the pcr product should be
284+1300+732 = 2316