Two Plasmids in One E coli

2015-01-13

azim58 - Two Plasmids in One E coli

Here's a few notes:

Notes from:http://bitesizebio.com/articles/plasmid-retention/

only a fraction of the cells are actually competent to take up bacteria
and a sub-population of those can readily take up two or more plasmids in
a single transformation.

26% of the cell population was transformable, and that only 10-20% of

those competent cells (so about 0.052%) of the population could take up
two or more plasmid molecules.
ONLY 0.052% OF THE CELL POPULATION CAN UPTAKE 2 PLASMIDS
up to 20% of your transformants could be double transformants.
Compatible (different) origins of replication are needed
It is not possible to maintain in a single cell two different plasmids
that use the same mechanism for replication.
Pasted from
<http://www.bio.davidson.edu/Courses/Molbio/Protocols/ORIs.html>