Tumor Lysate Nitrocellulose Print 9-6-12
2015-01-13azim58 - Tumor Lysate Nitrocellulose Print 9-6-12
The print will actually take place on 9-7-12
Print onto Grace Biolabs Nitrocellulose Slides (dimensions are on that
page)
The print will start about 5 mm down and 2.9 mm over. The pad starts
about 3 mm down and 2.5 mm over.
The print will cover about 53 mm down and 7.4 mm across. The pad covers
about 60 mm down and 20 mm over.
So everything should be able to fit.
Pin Operation
- Dip into peptide
- pretap 5X on one slide
- print 10 spots on 6 slides
- repeat pin cleaning 3X
o wash in 50% acetonitrile 10 s
o dry 5 s
- Sonicate 30 s
- wash 30 s
- dry 40 s
Note that each slide will have it's own "dip"
No landing lights used this time, but if they were, here is the
information for that:
Landing Light Concentration: 1:100
Landing Light Identity: a biotinylated peptide. Therefore, in order to
detect the landing lights, it is necessary to use streptavidin
AlexaFluor. If direct labeled secondary is used, the landing lights will
not be visible unless a small of amount (such as 0.1 nM) of streptavidin
AlexaFluor is added as well.