Tumor Library Nitrocellulose Print 2-27-12
2015-01-13azim58 - Tumor Library Nitrocellulose Print 2-27-12
Print onto Grace Biolabs Nitrocellulose Slides (dimensions are on that
page)
The print will start about 5 mm down and 2.9 mm over. The pad starts
about 3 mm down and 2.5 mm over.
The print will cover about 53 mm down and 7.4 mm across. The pad covers
about 60 mm down and 20 mm over.
So everything should be able to fit.
Pin Operation
- Dip into peptide
- pretap 5X on one slide
- print 1 spot on all 10 slides
- repeat pin cleaning 3X
o wash in 50% acetonitrile 10 s
o dry 5 s
- Sonicate 30 s
- wash 30 s
- dry 40 s
What is the concentration and identity of the landing lights?