Transformation to determine efficiency and complexity 10-25-12

2015-01-13

azim58 - Transformation to determine efficiency and complexity 10-25-12


Transformation to determine efficiency and complexity 10-25-12

Finished ethanol precipitating the in-fusion reaction started by Mara.

Here are the sample IDs

10-24-12 MM
Positive 18.1 ng/uL 1.61 260/280
Negative 20.8 ng/uL 1.85 260/280
Control A 30.4 ng/uL 1.68 260/280
Tumor A 40.0 ng/uL 1.67 260/280
Samples stored at Human tumor cDNA 8-3-12 -20 C

  1. 25 ng/(2 uL) = 0.125 ng/uL solutions were made from these to do a
transformation in which there would be about 500 colonies on a plate
spread with 50 uL solution if the transformation efficiency were 4e6
cfu/ug.