Thap_plus_Rbm chimeric transcript
2015-01-13azim58 - Thap_plus_Rbm chimeric transcript
Note that the rbm chimeric transcript is more appropriately called
Rbm14_Exon1_Rbm4b_Exon2
Original document from Shen which has the sequence information for this
chimeric transcript as well as the information for the Thap and Tmem
chimeric transcript
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\thap_plus_rbm\Mus CDS of THAP and Rbm.doc"
rbm_fus
GACAAGAGCCTACCACGTCAC
21bp
56.2 Tm
Rmb_fus and forward sequencing primer for pSMART2IF
resulting sequence 6-23-13d1836
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\thap_plus_rbm\psmart2IF with rbm_fus and forward screen.SPF"
Rmb_fus and reverse sequencing primer for pSMART2IF
resulting sequence 6-23-13d1839
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\thap_plus_rbm\psmart2IF with rbm_fus and reverse screen.SPF"
rbmF
CAATGTCGATGGGGCGGATA
20 bp
53.7 Tm
rbmR
CCACACCGATAGCAACCACT
20bp
53.7 Tm
primers last located at 4-26-13 -20 C chimeric transcript box
I also have some aliquots here: 7-2-13d1700 -20 C box
sequence from rbmF and rbmR
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\thap_plus_rbm\rbmF and rbmR.SPF"
possible primer pairs from primer blast
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\thap_plus_rbm\possible primer pairs from primer blast.txt"
Primer order form
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\Primer order form Kurt 6-23-13.xls"