Taq PCR Protocol
2015-01-13azim58 - Taq PCR Protocol
manual
"F:\kurt\storage\CIM Research Folder\DR\2013\5-16-13\Taq Polymerase.pdf"
http://biotech.wku.edu/online_manual/Taq%20Polymerase.pdf
TaqMaster buffer can also be used
TaqMaster is a buffer additive that improves thermostability (enzyme
half-life) and processivity of Taq DNA polymerase by stabilizing the
enzyme during PCR.TaqMaster makes the enzyme less sensitive to exogenous
PCR-inhibiting contaminants. TaqMaster is chemically stable, inert, and
does not change the optimal primer annealing temperature or the melting
behavior of template DNA.
"F:\kurt\storage\CIM Research Folder\DR\2013\5-16-13\mastertaq kit
manual_5prime_1044367_032007.pdf"
TaqMaster buffer should be heated at 65 C and vortexed vigorously until
completely dissolved before use and before storage.