TRF1 portal 09-25-2014d1413
2015-04-16TRF1 portal 09-25-2014d1413
Issues to investigate
- TRF1 overexpression
- TRF1 deletion
- also look at TRF2
Mice with decreased TRF1 expression live longer, and they don't know why. How much longer do they live? No expression seems to be very bad.
Good paper to look at
- TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells
- -recommended by Marinela
Some highlights
- A component of the shelterin complex
- Deficiency leads to embryonic lethality
- Deficiency leads to atrophy when deleted in adults
- Deficiency can impair tumor growth
- Highly expressed in stem cell compartments
- Highly expressed in iPSCs and also necessary for reprogramming
- TRF1 overexpression negatively regulates telomere length
- -cni_contradiction about TRF1 telomere length negative regulation and reprogramming 01-26-2015d1737
TRF1 papers
- Conditional TRF1 knockout in the hematopoietic compartment leads to bone marrow failure and recapitulates clinical features of Dyskeratosis congenita
- Increased telomere fragility and fusions resulting from TRF1 deficiency lead to degenerative pathologies and increased cancer in mice
- TRF1 controls telomere length and mitotic fidelity in epithelial homeostasis
TRF1 review papers
TRF1, a mammalian telomeric protein
1997
Telomere structure and telomerase in health and disease (Review) 2012
Telomerase and telomere-associated proteins: structural insights into mechanism and evolution.
2012
Shelterin complex and associated factors at human telomeres.
2011
Inside the mammalian telomere interactome: regulation and regulatory activities of telomeres.
2006;16(2):103-18. Review
Shelterin: the protein complex that shapes and safeguards human telomeres. de Lange
Regulation of telomerase by telomeric proteins.
Telomeres in cancer and ageing. Blasco 2011 paper
RefSeq Mouse Terf1 TRF1 RNA
NM_001286628.1
http://www.ncbi.nlm.nih.gov/nuccore/NM_001286628
Primers for TRF1 RNA
RflExonControl_Fw GTCTCTGTGCCGAGCCTTC TRF1 Schneider, 2013 110bp mouse (I don't understand where I got the name RflExonControl for these primers. In Ralph's paper they have the name mTRF1. I may have been confused.)
RflxExonControl_Rv TCAATTGGTAAGCTGTAAGTCTGTG TRF1 mouse
TRF1BF TCTAAGGATAGGCCAGATGCCA TRF1 Muñoz, 185 bp mouse
TRF1BR CTGAAATCTGATGGAGCACGT TRF1 mouse
eGFP—TRF1—F
CCTGAGCAAAGACCCCAAC
eGFP—TRF1—R
TCCTCCTGCTCTGGAGAATC
Some questions
What is the size of TRF1-eGFP protein?
- molecular weight is about 50 kD
- -see Figure 7C of western blot in Ralph Schneider's Thesis