T Cell Purification to Compare Old T Cell Enrichment Kits with New 1-4-12

2015-01-13

azim58 - T Cell Purification to Compare Old T Cell Enrichment Kits with
New 1-4-12


T Cell Purification to Compare Old T Cell Enrichment Kits with New 1-4-12
(files stored at 1-5-12)

Robosep Kit Used:
Negative Selection Mouse T Cell Enrichment Kit 19751 Lot 11F39752
Antibodies used for Flow Cytometry:
Product Information Sheets for Antibodies for Detecting T Cells 12-14-11

Protocols Followed:
Splenocyte Preparation Protocol
Purification of T cells with Robosep Kit
Antibody Staining Protocol. The product sheets for the antibodies don't
give very much information, but I would imagine the protocol would be
very similar to the Annexin-V antibody
Annexin V and PI or 7-AAD protocol
Flow Cytometry

o FS, Volts 635, Gain 5.0
o SS, Volts 431, Gain 50.0