Start process to construct transcript specific cDNA for blunt end cloning and sequencing
2015-01-13azim58 - Start process to construct transcript specific cDNA for blunt
end cloning and sequencing
Start process to construct transcript specific cDNA for blunt end cloning
and sequencing
associated plan
Plan for validating cDNA clones portal 1-7-13
Here are parts of the short names for the primers for the transcripts I
would like to amplify
RS61
RW17
RCX3
RRS8
REA
RR130
RR130WT
RH1
RM1
Rco1
refer to primers
Tumor Validation primers 1-28-13
I will just use much less of the 5' oligo to ensure that I have enough to
do the reaction (maybe I'll use 1/4).
===========================================================================
The PCR products were very smeared on the gel (see 2-13-12 of notebook).
I think I may need to use the RACE technique if I want to know the
sequences of these transcripts.