Slain2 chimeric transcript
2015-01-13azim58 - Slain2 chimeric transcript
original document from shen with the sequence that also shows where the
chimeric transcript is
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\Slain2\mus Slain2.doc"
Primer for Slain2 at the fusion site
slain_fus
TGGCAATGATTGGCTGAAGCC
21 bp
OligoMaster Tm: 54.2
primers last seen at 4-26-13 -20 C chimeric transcript box
I also have some aliquots here: 7-2-13d1700 -20 C box
slainF
CCTGCCCTTAATAGGTTTTCTCC
23bp
55.2 Tm
slainR
CTTTCCAACTGTCATCTTTCATGC
24bp
53.9 Tm
products from slain primer pairs
- sequence PCR amplified by slainF and slainR
- -Primer-Blast results for slainF and slainR
- sequence generated from slain fus and pSMART2IF forward screening primer
- sequence generated from slain_fus and reverse pSMART2IF primer
- pSMART2IF_for plus slain_fus
- pSMART2IF_for plus slainR
- slainF plus slain_fus
slain2 chimeric transcript in pSMART2IF vector
Primer order form
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\Primer order form Kurt 6-23-13.xls"
list of some sequencing results for slain primers portal 7-18-13