Shrimp Alkaline Phosphatase Promega
2015-01-13azim58 - Shrimp Alkaline Phosphatase Promega
Link to information sheet:
http://www.oardc.ohio-state.edu/tomato/604.03/Module1/promega%20shrimp%20al
kaline%20phosphatase.pdf
Promega SAP 1 u/uL (500 u in tube total) Cat # M820A or 25856303. Should
be stored at -20 C
SAP can inactivated at 65 C 15 min, whereas CAP (Calf Alkaline
Phosphotase) cannot be.
Main Points from Protocol
- 10 units SAP/μg vector in a final volume of 30–50μl. Stock is 1
- Incubate at 37°C for 15 minutes.
- Incubate at 65 C for 15 min
SAP Protocol