SfiI Site

2015-01-13

azim58 - SfiI Site

Restriction Site

GGCCNNNNNGGCC

Actual Sequence in PCANTAB plasmid
ggcccagccggcc

Unique part of Sequence: cagcc

Actual Sequence in scFv MHV_BACK_SfiI primer

GGCCCAGCCGGCC
Unique part

o CAGCC

Actual Sequence in pTriplEx2

SfiIA Site

o GGCCATTACGGCC
o Unique part: ATTAC
o Note that there was a discrepancy between the SMART cDNA library
and the pTriplEx2 vector sequence information. After contacting
Clontech, they responded (12-6-11) and informed me that they think
the user manual has the correct sequence. So "ATTAC" is the correct
sequence.

SfiIB Site

o GGCCATTACGGCC
o Unique part: GCCTC