Second Mouse CTL Experiment

2015-01-13

azim58 - Second Mouse CTL Experiment


Details of Dec-Jan 2012 Ova Immunization
"C:\Users\kurtw_000\Documents\kurt\storage\CIM Research Folder\DR\2014\01-18-2014d1351\wikispaces download 01-18-2014d1351\01-18-2014d1452\Dec_2011.jpg"
"C:\Users\kurtw_000\Documents\kurt\storage\CIM Research Folder\DR\2014\01-19-2014d1132\01-19-2014d1338\jan_2012.jpg"
Record started 12-20-11


o Dec. 22nd and 23rd (Thurs and Fri): Genetic Immunization with 2
bullets per mouse with 1ug pCMVi-LS Ova
- Is this 1 ug per bullet?
- I couldn't find whether we used CpGs the first time or not

o Jan. 5th: Protein Boost
o Jan 19th: Obtain Spleen
o
o

o Genetic Immunization 12-22-11 (I think; Andrey and Felicia did
this while I was gone)
o Genetic Immunization 12-23-11 (I think; Andrey and Felicia did
this while I was gone)
o Protein boost 1-10-12
o Protein boost 1-16-12
o Protein boost and cheek bleed 3-30-12
o 4-5-12 Cheek bleed
4-6-12 Cardiac puncture and splenectomy

o The adjuvant is currently suspended in PBS. I think there is
about 700 uL left.
o Saline solution of SAS stable up to 60 days. We suspended it on
10-13-11, and it expired on 12-12-11
o Example resuspension of SAS: just add 1 mL PBS and then mix this
1:1 with antigen. Remaining solution can last 60 days.
o 200 uL per mouse intraperitoneally
o Example solution: 0.05 mg/mL protein of interest and 0.5 mg/mL
SAS solution (all in PBS)
o Recommended Antigen concentration range is 0.05-0.25 mg/mL saline
o http://azim58.wikispaces.com/Sigma+Adjuvant+System




===========================================================================
Mice from older cage:

o 5 female BALB/c mice, Protocol 1000 R
o received 4 weeks old on 6-14-11
o Current age as of 12-22-11: 6 months 8 days
o Photo of Cage Card 2-9-12
o Cage card ending on 4-6-12
- Ova mouse end 1 4-6-12.pdf
- Ova mouse end 2 4-6-12.pdf