Screen of increasing tumor spot 6-17-12
2015-01-13azim58 - Screen of increasing tumor spot 6-17-12
I will pick one of the increasing tumor spots from this
experiment:Validation of most significant spots 6-1-12
(analysis found here: L:\storage\CIM Research Folder\DR\2012\6-11-12)
to expand out onto a new nitrocellulose array.
Spot chosen:
2-17-12_RP_16E3
2.9269E-18
1.179002009
Here is the general protocol I plan to follow in order to expand the well
out onto a new array.
Titration, Dilution, and Separation
Grow culture overnight (maybe 10 uL culture to 5 mL LB/carb).
Titrate this culture onto LB/Carb plates
Place remaining culture at 4 c.
Calculate transformation efficiency the next day
Dilute culture at 4 c so that there is 1 cfu/(200 uL)
Aliquot samples into wells of 96 well plates for overnight culture
Incubate plates in overnight at 37 C in HiGro shaker
Protein Production in Plate Format