Scalebars for CKX41 microscope

2015-01-13

azim58 - Scalebars for CKX41 microscope


Scalebars for CKX41 microscope images
40X, 100X, 200X, 400X
C:\kurt\storage\CIM Research Folder\DR\2013\1-17-13\data_download\2005

Notes about creation of these scale bars below

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Note that in order to use these scale bars, the magnification of the
camera should just be 1X.

I'd like to make black bars that can be used as scalebars in images from
the CKX41 microscope. In order to do this, I will need an example image
from the microscope, and then I will need to determine the dimensions.

Magnifications of Microscope: 40X, 100X, 200X, 400X


Example Microscope Image when converted from the original raw file to a
png file 12-13-11

This image has dimensions of 3087 X 2310 pixels (width X height) which
corresponds to 1089.02 X 814.92 mm.
Here's a table with some conversions of the different scales.


Magnification
Dimension mm
Dimension in um
Size of 100 um scale bar width in image (mm)

40X
27.2255 X 20.373
27,226 X 20,373
4

100X
10.8902 X 8.1492
10,890 X 8,149
10

200X
5.4451 X 4.0746
5,445 X 4,075
20

400X
2.72255 X 2.0373
2,723 X 2,037
39.994

Magnification column is basically just a known label
Dimension column is simply the normal numbers divided by the
magnification.
"Dimension in um" column just multiplies the numbers in the dimension
column by 1000
"Size of 50 um scale bar width in image" column multiplies 100 um by
1089.02 mm divided by the width at that magnification in um

1,089.02 mm/(27,226 um) =