Rnf130
2015-01-13++ Rnf130
alignments
- "F:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis\21G4 aka 21\rnf130 alignment.SPF"
- "F:\kurt\storage\CIM Research Folder\DR\2013\3-18-13\tumor sequence validation\Rnf130 primer alignment.SPF"
- "F:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis\21G4 aka 21\Rnf130 alignment.ape"
Primers for amplifying Rnf130
RR130 TTTTTGCTTAGCAACACGGTACTTTTATTTTATT
RR130Tr CGCTTGTGTGGCATGATTGG
RR130WT TTTTTATTTTATTATTTATTTATTTTAATACAAAGCTTTGGC
FR130 gccccgcctctcagcac
FR130Tr CTGACTTGCAGCCTGTGGC
FR130Tr2
cgagcggacaacgcgag
17 bp
54.1 Tm
RR130Tr2
CTTCAAGGCAATCAGTCAGAAAGC
24 bp
55.6 Tm
FR130Tr+RR130Tr (1298 bp) -> FR130Tr2+RR130Tr2 (1223 bp)