RAW cells taken from freezer 11-29-11

2015-01-13

azim58 - RAW cells taken from freezer 11-29-11


Cell Type: RAW 264.7
macrophages from BALB/c ascites transformed with Abelson murine leukemia
virus
complete DMEM should be used
Don't use trypsin
Subcultivation ratio: 1:3 to 1:6 is recommended
Medium Renewal replace every 2 to 3 days


Original vial in freezer was from Carlos


Note that Kary has also ordered some fresh RAW 264.7 which we could have
used, but we did not.

We thawed the vial at 37 C, placed in ethanol. We then placed the cells
in about 13 mL complete DMEM media. Note that the cells were centrifuged
and resuspended to remove DMSO.


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11-30-11 10 more mL complete DMEM was added.