Quantification of bands 6-24-13d1006
2015-01-13azim58 - Quantification of bands 6-24-13d1006
top band mean intensity
44.2
middle band mean intensity
102.2
bottom band mean intensity
114.8
empty part of gel
148
top band of ladder mean intensity
23.8
Band values inverted
top band mean intensity
148-44.2=103.8
middle band mean intensity
148-102.2=45.8
bottom band mean intensity
148-114.8=33.2
empty part of gel
148-148=0
top band of ladder mean intensity
148-23.8=124.2
10 kb band is 36 ng when 20 uL is loaded. I loaded 12 uL so there was
36*"ng"/20*"uL"*(12*"uL")=21.6*"ng"
Therefore my conversion factor from amount to intensity is
21.6*"ng"/(124.2*"i")
top band amount
103.8*"i"*21.6*"ng"/(124.2*"i")=18.1*"ng"
so 18.1*"ng"/(10*"uL")=1.8*"ng"/"uL"
middle band amount
45.8*"i"*21.6*"ng"/(124.2*"i")=7.97*"ng"
so 7.97*"ng"/(10*"uL")=0.8*"ng"/"uL"
bottom band amount
33.2*"i"*21.6*"ng"/(124.2*"i")=5.8*"ng"
so 5.8*"ng"/(10*"uL")=0.6*"ng"/"uL"