Protein Production for printing 8-31-12

2015-01-13

azim58 - Protein Production for printing 8-31-12


Items

o see 8-26-12 Plasmids for verification
o Layout of Plate



1
2
3
4

A
81
33
42


B
41
31
21


C
11
4
71


D
62
61
51
PUC19

created 2 more plates containing 50 uL of protein solution. One plate is
the reverse of the other for printing purposes. Also e coli lysate was
used instead of PUC19 for the negative control.
Tumor Proteins 8-31-12_2 KW (in 384 well plate)


1
2
3
4

A
81
33
42


B
41
31
21


C
11
4
71


D
62
61
51
E coli



Tumor Proteins 8-31-12_3 KW (in 384 well plate)


1
2
3
4

A
E coli
51
61


B
62
71
4


C
11
21
31


D
41
42
33
81



for verification)
o Soluble Protein 1 8-31-12
- from top row to bottom row: 81, 33, 42, 41, 31, 21, 11, 4

o Soluble Protein 2 8-31-12
- from top row to bottom row: 71, 62, 61, 51, PUC19


o Insoluble protein 1 8-31-12
- from top row to bottom row: 81, 33, 42, 41, 31, 21, 11, 4

o Insoluble protein 2 8-31-12
- from top row to bottom row: 81, 33, 42, 41, 31, 21, 11, 4