Printing Actual Library 010912

2015-01-13

azim58 - Printing Actual Library 010912

Planning to Print Actual cDNA Library 122911

Protocol for Printing cDNA Library 1-9-12

Printing small or individual pools portal 4-11-12

Items 1-21-12

Plate Batches

Tumor Library Lysate 1-8-12 (2*96+8)
Tumor Library Lysate 1-11-12 (3*96+82=370 wells)
4T1 cDNA Library Protein Production in Plate Format for all 3

ligations 1-12-12 (36+60+29 = 125 wells)

Protein Production in plate format 1-19-12 (96+7*12+4 = 184)
Tumor Library Lysate 1-21-12 ((2*96+12+9=213) + 12 PUC19 rows)
Tumor Library Lysate 1-26-12 (96*2+4*12+2 = 242 + 12

pGEX-SMC1fs-27mer wells)

Tumor Library Lysate 2-1-12 plate (96*5+12*6+5 =557)
Tumor Library Lysate 2-17-12: 1.73e6 colonies (96*17+12*5+11 = 1703)
Note that there are some plates that have a date past 2-17-12 (I

think it might be 2-21-12 or something). This was just a mistake in
labeling. These 2-21-12 plates belong to the 2-17-12 batch.
Current well count

(2*96+8)+(3*96+82)+(36+60+29)+(96+7*12+4)+(2*96+12+9)+(96*2+4*12+2)+(96

*5+12*6+5)+(96*17+12*5+11)=3594
Current colony count

(2*96+8)*946.8+(3*96+82)*1000+(36+60+29)*1000+(96+7*12+4)*1000+(2*96+12

+9)*1000+(96*2+4*12+2)*1000+(96*5+12*6+5)*1000+(96*17+12*5+11)*1000=3.58e
6
see All Glycerol Stock for Tumor Library 2-17-12

Cell type used for transformations 8-27-12
Check for Protein 2-13-12
Transfer of Lysate to 384 well plates 2-23-12
Tumor Library Nitrocellulose Print 2-27-12
Approximate Concentrations of Tumor cDNA Library Glycerol Stocks
Biomek
HiGro

Map of Experiments
4T1 cDNA Library Transformation 1-6-12
4T1 cDNA Library Protein Production in Plate Format 1-7-12
4T1 cDNA Library Protein Production in Plate Format 1-8-12
Single Electroporation 1-9-12
4T1 cDNA Library Protein Production in Plate Format 1-11-12
4T1 cDNA Library Protein Production in Plate Format for all 3 ligations
1-12-12
Five Transformations of A Ligation 1-17-12
Protein Production in plate format 1-19-12
Check for Protein 2-13-12
Colonies on Plates 2-16-12