Primers to check for scFv

2015-01-13

azim58 - Primers to check for scFv

Primers to check for scFv

To check a kappa insert, use these primers

o MHV_Back_SfiI + MKV_For_NotI

To checka lambda insert, use these primers

o MHV_Back_SfiI + MLV_For_NotI

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MHV_BACK_SfiI
19 bp
CATATGGCCCAGCCGGCCA
58 basic Tm
52 salt-adjusted Tm
62 base-stacking Tm
68 % GC

Tm calculated with
http://www.promega.com/techserv/tools/biomath/calc11.htm#melt_results

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MKV_FOR_NotI
18 bp
GTTCTTGCGGCCGCCCGT
57 basic Tm
52 salt-adjusted Tm
64 base-stacking Tm
72 % GC

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MLV_FOR_NotI
18 bp
GTTCTGCGGCCGCCTTGG
57 basic Tm
52 salt-adjusted Tm
62 base-stacking Tm
72 % GC