Primers for amplifying SMC1fs

2015-01-13

azim58 - Primers for amplifying SMC1fs


100 uM stock primers last seen at (newest to oldest)
7-2-13d1700 -20 C box
Tumor Library pools 4-9-13 -20 C box
I also have some aliquots here: 7-2-13 -20 C box

SMC1-mou-RV
Tm 55.6
OligoMaster calculated Tm: 49.3
17 bp
GAGCTGTCCTCTCCTTG

SMC1-mou-FWD
Tm 54.9
OligoMaster Calculated Tm: 46.9
17 bp
CTGTCATGGGTTTCCTG

SMC1 WT Size: 464 bp
SMC1fs Size: 162 bp

Products on potentially unintended templates
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\unintended smc1fs
pcr products.txt"


products for some primer pairs



some sequencing results for smc1-mou-FWD and SMC1-mou-RV portal 7-17-13

smc_fus primer