Primers for Constructing scFv

2015-01-13

azim58 - Primers for Constructing scFv


LINK_HEAVY
40 bp
CAGAGCCACC TCCGCCT GAACCGCC TCCACCT GAGGAGAC
88 Tm
68 % GC

LINK_LIGHT
34 bp
TTCAGGCGGAGGTGGC'TCTGGCGGTGGCGGAICG
90 Tm
71 % GC



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MHV_BACK_SfiI
19 bp
CATATGGCCCAGCCGGCCA
58 basic Tm
52 salt-adjusted Tm
62 base-stacking Tm
68 % GC

Tm calculated with
http://www.promega.com/techserv/tools/biomath/calc11.htm#melt_results




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MKV_FOR_NotI
18 bp
GTTCTTGCGGCCGCCCGT
57 basic Tm
52 salt-adjusted Tm
64 base-stacking Tm
72 % GC




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MLV_FOR_NotI
18 bp
GTTCTGCGGCCGCCTTGG
57 basic Tm
52 salt-adjusted Tm
62 base-stacking Tm
72 % GC