Primers Used for 1st Construction of scFv Genes

2015-01-13

azim58 - Primers Used for 1st Construction of scFv Genes

NotI Site: GCGGCCGC
SfiI Site: GGCCCAGCCGGCC

see also Table of primers used for scFv construction (note that I'm not 100% sure the primers in this table that were used are the same as the primers on this page since I added this note at this time: 12-09-2013d2051)

"C:\Users\kurtw_000\Documents\kurt\storage\CIM Research Folder\DR\2013\12-9-13\Table of primers used for scFv construction 12-09-2013d2052.xlsx"

MHV_BACK1
CATATGGCCCAGCCGGCCATGGCCGATGTGAAGCTTCAGGAGTC

MHV_BACK2
CATATGGCCCAGCCGGCCATGGCCCAGGTGCAGCTGAAGGAGTC

MHV_BACK3
CATATGGCCCAGCCGGCCATGGCCCAGGTGCAGCTGAAGCAGTC

MHV_BACK4
CATATGGCCCAGCCGGCCATGGCCCAGGTTACTCTGAAAGAGTC

MHV_BACK5
CATATGGCCCAGCCGGCCATGGCCGAGGTCCAGCTGCAACAATCT

MHV_BACK6
CATATGGCCCAGCCGGCCATGGCCGAGGTCCAGCTGCAGCAGTC

MHV_BACK7
CATATGGCCCAGCCGGCCATGGCCCAGGTCCAACTGCAGCAGCCT

MHV_BACK8
CATATGGCCCAGCCGGCCATGGCCGAGGTGAAGCTGGTGGAGTC

MHV_BACK9
CATATGGCCCAGCCGGCCATGGCCGAGGTGAAGCTGGTGGAATC

MHV_BACK10
CATATGGCCCAGCCGGCCATGGCCGATGTGAACTTGGAAGTGTC

MHV_FOR1
GCCTCCACCTGCAGAGACAGTGACCAGAGT

MHV_FOR2
GCCTCCACCTGAGGAGACTGTGAGAGTGGT

MHV_FOR3
GCCTCCACCTGAGGAGACGGTGACTGAGGT

MHV_FOR4
GCCTCCACCTGAGGAGACGGTGACCGTGGT

MKV_BACK1
TCTGGCGGTGGCGGATCGGATGTTTTGATGACCCAAACT

MKV_BACK2
TCTGGCGGTGGCGGATCGGATATTGTGATGACGCAGGCT

MKV_BACK3
TCTGGCGGTGGCGGATCGGATATTGTGATAACCCAG

MKV_BACK4
TCTGGCGGTGGCGGATCGGACATTGTGCTGACCCAATCT

MKV_BACK5
TCTGGCGGTGGCGGATCGGACATTGTGATGACCCAGTCT

MKV_BACK6
TCTGGCGGTGGCGGATCGGATATTGTGCTAACTCAGTCT

MKV_BACK7
TCTGGCGGTGGCGGATCGGATATCCAGATGACACAGACT

MKV_BACK8
TCTGGCGGTGGCGGATCGGACATCCAGCTGACTCAGTCT

MKV_BACK9
TCTGGCGGTGGCGGATCGCAAATTGTTCTCACCCAGTCT

MKV_FOR1
GTTCTTGCGGCCGCCCGTTTCAGCTCCAGCTTG

MKV_FOR2
GTTCTTGCGGCCGCCCGTTTTATTTCCAGCTTGGT

MKV_FOR3
GTTCTTGCGGCCGCCCGTTTTATTTCCAACTTTG

MLV_BACK
CAGGCTGTTGTGACTCAGGAA

MLV_FOR
GTTCTGCGGCCGCCTTGGGCTGACCTAGGACAGT

MLV_BACK
TCTGGCGGTGGCGGATCGCAGGCTGTTGTGACTCAGGAA

Linker light sequence
TTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCG

Linker heavy sequence
CAGAGCCACCTCCGCCTGAACCGCCTCCACCTGAGGAGAC

MHV_BACK_SfiI: CATATGGCCCAGCCGGCCA

MKV_FOR_NOTI:
GTTCTTGCGGCCGCCCGT

MLV_FOR_NOTI:
GTTCTGCGGCCGCCTTGG