Primer order for random hexamer library 8-28-12
2015-01-13azim58 - Primer order for random hexamer library 8-28-12
Since there could be stop codons in the 5' UTR region of full length
mRNAs, we will make the human tumor cDNA library using random hexamer
primers. The beginning of these random hexamer primers will contain the
In-Fusion site necessary for ligating the genes.
pSMART2IFD in-fusion site information
left side of in-fusion
CGGGGTACGATGAGA
right side of in-fusion
TTGATACCACTGCTT
reverse complement of right side of in-fusion
AAGCAGTGGTATCAA
original thoughts on ordering 8-28-12
random hexamer primers for human tumor cDNA library