Preparing Complete Tissue Culture Media
2015-01-13azim58 - Preparing Complete Tissue Culture Media
Preparing Complete Tissue Culture Media
Add 100X penicillin/streptavidin to media. Add 10 mL to 1000 mL media.
Add 100 mL FBS (fetal bovine serum) to 1000 mL media for 10% FBS
New media last seen in 4 C walk in fridge
penicillin/streptavidin last seen in -20 fridge in tissue culture room
FBS last seen in -20 fridge in tissue culture room
Some media contains sodium pyruvate. According to wikipedia
(http://en.wikipedia.org/wiki/Sodium_pyruvate), sodium pyruvate is
commonly added to cell culture media as an additional source of energy,
but may also have protective effects against hydrogen peroxide.