Preparation of a Linearized Vector by PCR
2015-01-13azim58 - Preparation of a Linearized Vector by PCR
PrimeStar Max polymerase seems to work the best for vector linearization
see 11-27-12 Polymerase system test for polymerization
for initial test of polymerases for linearization
1 ng of template seems to work just fine
see 11-27-12 of notebook for an example
===========================================================================
Advantage HD can also be used, but doesn't seem to work as well
see
"C:\kurt\storage\CIM Research Folder\DR\2012\11-5-12\preparation of a
linearized vector by PCR 11-5-12.docx"
The In-Fusion SMARTer Directional cDNA Library Construction Kit
also has instructions for linearization.