Practice isolating stem cells from mouse 6-5-12

2015-01-13

azim58 - Practice isolating stem cells from mouse 6-5-12

searched for protocol for isolating cells from bone marrow

followed Mouse Bone Marrow Isolation Protocol

Pictures of cells before and after robosep purification

o S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data
Often Originally on Research Drive\2012\6-7-12

Annotated

o L:\storage\CIM Research Folder\DR\2012\6-7-12

38e6 cells from an original 6.44e7 cells were isolated from this

experiment.

Message to Kathy about experiment
https://mail.google.com/mail/u/0/?ui=2&shva=1#sent
She thought I should store the cells in trizol.

Cells were pelleted and stored in 500 uL trizol, and stored at -80 C
Negative fraction progenitor cells 6-8-12 stored at -80 C Kurt 5-23-10
Positive fraction bone marrow cells 6-8-12 stored at -80 C Kurt 5-23-10