Post print wash protocol

2015-01-13

azim58 - Post print wash protocol


This wash is based on the prewash procedure described on page 189 of Matt
Grevingç—´ dissertation. The formula does not remove the barcode. Prepare
  1. 33% acetonitrile, 33% isopropanol and 0.55% triflouroacetic acid in
nanopure water.


  1. Place slides in a slide washer occupying only every other space to
ensure good solvent flow.
  1. Submerge slides in wash buffer and place on the rotating platform
in the chemical fume hood that is set for slow speed (approximately
setting 4) for 5 min.
  1. Manually wash 1x in TBST.
  2. Manually wash 1x in ddH2O.
  3. Spin dry at 1500 rpm for 5 min.