Pierce Silver Stain Protocol

2015-01-13

azim58 - Pierce Silver Stain Protocol

Pierce Silver Stain Protocol
Item 24612
http://www.piercenet.com/instructions/2161478.pdf
Note that this pdf contains more information and a more in-depth protocol
than the protocol below

Wash gel 2 × 5 minutes in ultrapure water.

o Make sure to handle the gel with clean gloves or sterile
equipment to avoid later smudges on the gel

Fix gel 2 × 15 minutes in 30% ethanol:10% acetic acid solution.
Wash gel 2 × 5 minutes in 10% ethanol, then 2 × 5 minutes in

ultrapure water.

Prepare Sensitizer Working Solution (50μL Sensitizer with 25mL

water).

Sensitize gel for 1 minute, then wash 2 × 1 minute with water.

o Make sure that any silver stain reagents are discarded in an
appropriate silverstain waste container.

Prepare Stain Working Solution (0.5mL Enhancer with 25mL Stain).
Stain gel for 30 minutes.
Prepare Developer Working Solution (0.5mL Enhancer with 25mL

Developer).

Wash gel 2 × 20 seconds with ultrapure water, then develop gel for

2-3 minutes until bands appear.

Stop with 5% acetic acid for 10 minutes.