Pfu
2015-01-13azim58 - Pfu
Pfu Polymerase
This is a very high fidelity polymerase
last seen in MDR-Rack 4 (bottom row (MDR 49)) in Misc: Plasmid . . box
PCR PROTOCOL FOR PfuTURBO® DNA POLYMERASE
on pg. 12 (16 in pdf numbers) of
http://stanxterm.aecom.yu.edu/wiki/data/Product_manuals_attach/pfuturbo.pdf
Reaction Mixture
Distilled water (dH2O) 40.6 µl
10× cloned Pfu reaction buffera 5.0 µl
dNTPs (25 mM each dNTP) 0.4 µl
DNA template (100 ng/µl) 1.0 µlb
Primer #1 (100 ng/µl) 1.0 µlc
Primer #2 (100 ng/µl) 1.0 µlc
PfuTurbo® DNA polymerase (2.5 U/µl) 1.0 µl (2.5 U)d
Total reaction volume 50 µl
PCR Cycling Parameters
see manual