Overnight primarysecondary only Tecan Protocol
2015-01-13azim58 - Overnight primarysecondary only Tecan Protocol
Protocol
wash TBST 30 s
hybridization (for blocking buffer) 23 C 1 hr
Wash TBST 30 S
hybridization (primary) 23 C 16 hr
Wash TBST 30 s
hybridization 23 C 1 hr
Wash TBST 30 s
Wash H20 30 s
slide drying 30 C 5 min
Image of protocol from Tecan computer monitor
"C:\kurt\storage\CIM Research Folder\DR\2013\3-12-13\overnight primary
secondary only.jpg"