Ordering primers to verify sequence 10-25-12
2015-01-13azim58 - Ordering primers to verify sequence 10-25-12
Ordering primers to verify sequence 10-25-12
There were some odd things around where the NotI site was removed in the
pComb MN site, but this was towards the end of the sequence. I will order
some new primers to determine what this sequence is exactly.
see Plan for modifying pComb3XSS vector 061312
potential primer at 2362 of pcomb MN plasmid
MNVF (modified notI verification forward)
5' CTTCCCTCCCTCAATCGG 3'
Oligo Master Tm: 52.5 C
primer located at 2381
("C:\kurt\storage\CIM Research Folder\DR\2012\7-26-12\pcomb sequence
analysis\generating new pcomb sequence 7-28-12.SPF")
Reverse primer
MNVR (modified notI verification reverse)
CTCCAACGTCAAAGGGCG
appropriate reverse complement sequence
5' CGCCCTTTGACGTTGGAG 3'
Oligo Master Tm: 52.5 C
primer located at 2776 of pcomb MN
10-31-12 received pcomb validation primers