Numbers associated with pcomb rcomp ligation 10-26-12
2015-01-13azim58 - Numbers associated with pcomb rcomp ligation 10-26-12
The unmodified pComb3xSSv2 vector has the following features.
SfiI 275
NotI 1952
1952-275 = 1677
vector is 4973 bp
vector with fragment removed is 4973-1677 = 3296 bp
If the ligation worked properly, then the rCompTT fragment should be 1.3
kb