NotI restriction digest
2015-01-13azim58 - NotI restriction digest
This was a test of the NotI restriction digest to make sure that the
enzyme and conditions were working appropriately. The NotI digest was for
cutting the PCANTAB vector with SfiI and NotI to eventually insert scFv
fragments for a phage antibody library. The NotI enzyme seemed to work
just fine.
An in-gel stain and a post stain were also compared.
Amount of PCANTAB Cut = 800 ng
Amount of Uncut PCANTAB used = 100 ng
Amount of rCompTT Cut = 1000 ng
Amount of uncut rCompTT = 100 ng
The plasmids that were cut were cut with NotI only.
Two identical gels were made. One for the in-gel stain and one for the
post-stain.
Sample order: 100 bp GeneRuler Fermentas Cut PCANTAB Uncut PCANTAB Cut
rCompTT Uncut rCompTT
In-gel Stain
Post-Stain