Nested pcr screen scheme for frameshift sequences in plasmid 7-31-13
2015-01-13azim58 - Nested pcr screen scheme for frameshift sequences in plasmid
7-31-13
I plan to screen the pools with a forward vector primer + reverse
transcript primer. Then I will do the nested pcr with the forward
transcript primer followed by the fus primer.
I plan to screen the cDNA with a forward transcript primer + reverse
transcript primer. Then I will do the nested pcr with the forward
transcript primer followed by the fus primer.
I think I will primarily do these screens with smc and slain
pSMART2IF_for plus slainR (1502 bp)
slainF plus slain_fus (362 bp)
sequence PCR amplified by slainF and slainR (405 bp)
pSMART2IF_for plus SMC1-mou-RV (163 bp)
smc_fus plus SMC1-mou-FWD (124 bp)
Primers for amplifying SMC1fs (162 bp)