Meeting with Stephen 4-2-13
2015-01-13azim58 - Meeting with Stephen 4-2-13
e-mail to Kathy
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I met with Stephen today about my presentation tomorrow.
He is extremely focused on determining whether the SMC1fs transcript was
in my cDNA library and whether it is on my array. I know I have some old
data on this that I'll have to dig up, but it looks like I may also need
to do some further investigation as well.
He also thinks making a new library may not be necessary since the
frameshift information is at the end of the transcript anyway which is
what I have. He thinks that if I really want to pursue making another
library that I need to quantitatively prove that it is really necessary
to create a new library to find what I want. I'll have to spend some time
thinking about this.
Perhaps I could just find the clone on the array that has SMC1fs
naturally, deconvolute that to the single clone, and be finished with
graduate work with that set of experiments.
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To do list from meeting
read dissertation of Muskan
look at representation of library
see if several peptides all line up with the exact same part of the
transcript/protein sequences of the proteins rather than the distribution
of alignments
compare HER2 with 4T1 signature